5th International Workshop for Mangrove Biodiversity Studies by eDNA Metabarcoding

7 - 24 December, 2023
at Iriomote Station, TBRC, University of the Ryukyus

Co-sponsored by JSPS Core to Core, e-ASIA JRP and KAKENHI International (B)

Welcome to Iriomote Station!

We are pleased to hold the “5th International Workshop for Mangrove Biodiversity Studies by eDNA Metabarcoding” at Iriomote Station, Tropical Biosphere Research Center (TBRC), University of the Ryukyus, Japan. This workshop is in conjunction with a class at Graduate School of Agriculture, University of the Ryukyus "熱帯植物多様性特論I・II."

The international collaboration project for eDNA research on global mangroves started in 2020 by obtaining support from JSPS Core to Core, but we haven't had any face-to-face meetings with coordinators because of the outbreak of Covid-19.

We will explore the use of eDNA metabarcoding for biodiversity monitoring, in this case, not only for fish but also for plants with workshop participants from Indonesia, Philippines, Malaysia, and Senegal. In one week intensive course, we explore the basics and advances of eDNA metabarcoding.

Workshop Schedule

7 - 12 December: Data analyses and paper production workshop with Mohammad Basyuni (University Sumatera Utara, Indonesia).
13 - 14: Field sampling and preparation for the workshop
15 - 17: Planning for the study subjects in the workshop and DNA extraction
18: Field Work at Nakama River Mouth
19: Field visit to Pinai-sara Water Fall and sample collection
20 - 22: DNA extraction, 1st and 2nd PCR for library preparation, iSeq100 Sequencer run
23: Data analyses session
24 Closing :

Organizers

Tadashi Kajita (TBRC, University of the Ryukyus, Japan; ITBC, Universiti Malaysia Sabah, Malaysia)
Yukinobu Isowa (TBRC, University of the Ryukyus, Japan), Secretary
Cecilia Chu (TBRC, University of the Ryukyus, Japan), Lab work manager
Bernadeth Grace Pananganan, Teaching Assistant
Saki Sugawara, Teaching Assistant

Participants

Chen Ann Cheng (Borneo Marine Institute, Universiti Malaysia Sabah, Malaysia)
Mohammad Basyuni (Universiti Sumatera utara, Indonesia)
Jean Fall (Cheik Anta Diop University, Senegal / TBRC, University of the Ryukyus, Japan)
Marizka Juliano (Gent University, Belgium)
Chris Ng Wei-Ling (Borneo Marine Institute, Universiti Malaysia Sabah, Malaysia)
Parivaseni Ravichandran (Borneo Marine Institute, Universiti Malaysia Sabah, Malaysia)
Shuhei Yoshioka (Graduate School of Agriculture, University of the Ryukyus, Japan)

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