1st International Workshop for Mangrove Biodiversity Studies by eDNA Metabarcoding

19 - 26 May, 2022
at Iriomote Station, Tropical Biosphere Research Center, University of the Ryukyus, Japan

Sponsored by JSPS Core to Core, e-ASIA JRP, CRRP Malaysia, and KAKENHI International (B)

Welcome to Iriomote Station!

We are pleased to hold the “1st International Workshop for Mangrove Biodiversity Studies by eDNA Metabarcoding” at Iriomote Station, Tropical Biosphere Research Center (TBRC), University of the Ryukyus, Japan.

The international collaboration project for eDNA research on global mangroves started in 2020 by obtaining support from JSPS Core to Core, but because of the outbreak of Covid-19, we haven't realized face-to-face activities. Under the difficult situation of the pandemic, we held online symposiums, seminars, and training courses, and sought the chances to expand the international collaboration. Our efforts bore fruits, and we newly obtained international research grants, CRRP Malaysia, e-ASIA JRP, JSPS KAKENHI Fostering Joint International Research (B), and AJ-CORE JRP. Our international collaboration to study the biodiversity of the global mangrove ecosystem has a step forward to the next stage!

By obtaining support from these projects, we hold the first face-to-face after the long Covid-19 restriction for international travel. We started this event with five Asian countries that allow international travel of participants. In the one-week workshop, we hold lectures, seminars, and training courses for eDNA analyses. We still have various Covid-19 restrictions and difficulties to perform face-to-face workshops, but we will do our best. We hope this workshop will be good practice for us to hold another one in near future.

Program

19 May: 14:00 - 17:00. Opening. Meeting with all members. Lecture: overall guidance for the eDNA metabardocing study and Practice

20 May: 9:00 - 17:00. Morning: Visit nearby mangrove and water sampling practice in mangrove field. Afternoon: DNA extraction from water samples. Lecture and Practice

21 May: 9:00 - 17:00.Lecture and Practice: 1st PCR and purification

22 May: 9:00 - 17:00.Lecture and Practice: 2nd PCR and E-Gel purification. Library Preparation.

23 May: 9:00 - 17:00. Morning: NGS Sequencing run; Afternoon. Mangrove field visit

24 May: 9:00 - 17:00. Lecture and Practice: Data analyses

25 May: 9:00 - 17:00. Lecture and Practice: Data analyses

14:00 JSPSC2C Seminar. Susumu Hyodo (Atmosphere and Ocean Research Institute, The University of Tokyo). "The study of spatiotemporal
distribution of juvenile chum salmon by application of an improved protocol for species-specific environmental DNA detection"

15:00 JSPSC2C Seminar.

  • Tadashi Kajita (Tropical Biosphere Research Center, University of the Ryukyus). "Introduction of eDNA metabarcoding data for Fish and Crustacean Diversity from mangrove ecosystem"

  • Alison Wee (University of Nottingham, Malaysia), "Development of environmental DNA biomonitoring methodology for coastal resource management of Southeast Asian mangroves"

  • Mohammad Basyuni (Universitas Sumatra Utara, Indonesia), "Monitoring biodiversity of fish, macrobenthos and plants in Indonesian mangroves using DNA barcode and metabarcoding"

  • Venus Leopardas (Mindanao State University at Naawan, Philippine), "Application of eDNA metabarcoding in faunal biodiversity assessment of Indo-Pacific mangroves vulnerable to climate change"

26 May: 9:00 - 11:00. Closing.

Organizers

Tadashi Kajita (University of the Ryukyus, Japan ), Chairperson

Yukinobu Isowa (University of the Ryukyus, Japan), Secretary

Mohammad Basyuni (Universitas Sumatra Utara, Indonesia), Co-Chairperson

Alison Kim Shan Wee (University of Nottingham, Malaysia), Co-Chairperson

Venus Leopardas (Mindanao State University at Naawan, Philippine), Co-Chairperson

Itchika Sivaipram (Chulalongkorn University, Thailand), Co-Chairperson

Supporters

Tropical Biosphere Research Center, University of the Ryukyus (Japan)

University of Nottingham (Malaysia)

Universiti Malaysia Sabah (Malaysia)

Universitas Sumatra Utara (Indonesia)

Mindanao State University at Naawan (Philippines)

University of the Philippines Diliman (Philippines)

Chulalongkorn University (Thailand)

The United Graduate School of Agricultural Sciences, Kagoshima University

The University of Tokyo

Sponsors

JSPS Core to Core Program.

Program HP: https://www.jsps.go.jp/j-c2c/, Project HP: http://c2c.mangroves.info
(No. JPJSCCB20200007)

e-ASIA JRP

Program HP: https://www.the-easia.org/jrp/ Project HP (information) : https://www.jst.go.jp/inter/english/program_e/multilateral_e/e_asia.html

Asia-Pacific Network for Global Change Research.

Program HP: https://www.apn-gcr.org/, Project HP (information) : https://www.apn-gcr.org/project/development-of-environmental-dna-reference-database-for-coastal-resource-management-of-southeast-asian-mangroves/

JSPS KAKENHI - Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)) for FY2022

Program HP: https://www.jsps.go.jp/j-grantsinaid/35_kokusai/04_kyoudoub/download.html