• 6 January - 8 February:  Participants' Arrival at Iriomote Station.  

  • Researchers and Students from five countries work on their projects using the eDNA lab facilities of Iriomote Station. They will get help, support, and advice from the staff of the University of the Ryukyus to conduct their research. 

• 9 February:  International Symposium on Mangrove biodiversity study by using eDNA metabarcoding

• 10   morning: Symposium (cont).   Afternoon: Excursion

• 11 - 13:  Library preparation for eDNA metabarcoding and  NGS run by Illumina iSeq100 with Data Analyses session

• 14   Closing  the workshop

9th February 

• 10:00 - 

  • Opening Remarks by Tadashi Kajita

  • Michio Kondoh: Using time-series data for better understanding of complex ecosystem

  • Imane Sioud: Advancing Biodiversity Monitoring through Collaboration: ANEMONE Global's Roadmap Ahead

  • Yoshihisa Suayam:   Identifying Genetic Disturbances in Malaysian Mangrove Restoration with Genome-wide SNP Analysis

  • Leanne Frances Yee: Surveying ichthyofaunal diversity using eDNA metabarcoding in the mangrove estuaries of Peninsular Malaysia

  • Heng Wei Khang: Integrading eDNA and Drones for Monitoring Marine Megafauna: A Dugong Case Study

  • 11:30 - Visit Supermarket to buy Lunch

• 13:00-

  • Mohammad Basyuni: Assessing Fish biodiversity in Indonesian mangrove ecosystems using eDNA Metabarcoding

  • Jean Fall: Introduction of AJ-Core project and recent DNA barcoding study in Senegal 

  • Codou Mbaye: Study of fish diversity using eDNA metabarcoding in the Sine Saloum island Casamace and Saint-Louis

  • Marizka Juliano:  Environmental DNA (eDNA) metabarcoding as a tool for fish biodiversity monitoring and resource management in Japan's largest mangrove forest: The Nakama River, Iriomote Island, Okinawa, Japan

  • Bernadece Pananganan:  Application of eDNA metabarcoding for faunal biodiversity assessment in mangrove ecosystem

  • Discussion and suggestions for the presentation by students.

  • Tea Break

• 15:00

  • James Reimer: Phylum Cnidaria: Subphylum Anthozoa - eDNA reference collections

  • Ulla von Ammon: My eDNA journey or sequence everything, everywhere, all at once

  • Fabian Gosser: Rubble in Paradise: Testing out different methods for metabarcoding of coral rubble communities

  • Yuki Minegishi: Ecology of chum salmon: Juvenile with environment DNA

  • 18:00 Dinner

• 19:00

  • Workshop discussion on the use of eDNA and other NGS based methods for biodiversity monitoring (AJ-Core)

• 21:00:    Closing 1st Day

10th February

• 9:00   Meeting with people who work on biodiversity conservation of world heritage site in Iriomote

  • Introduction of conservation activities in Iriomote Island (including eDNA use): Facilitated by Tadashi Kajita

    • Iriomote Wildlife Conservation Center (IWCC), Keisei Soma: introduction of conservation efforts in Iriomote Island

    • Progress report and brief introduction of biodiversity monitoring by eDNA metabarrcoding in Iriomote Island.  (Bernadeth, Marizka, Shiromoto, Kajita) (in Japanese and English

  • Discussion for future collaboration

• 11:00: Start to Urauchi River by car

  • Visit the supermarket on the way to buy lunch.

  • Mangrove site visit of Urauchi River (Badeth)

  • Lunch 

• 12:30 Urauchi River Cruise

  • 13:00 Start trekking

  • 14:30 Kampire Water Fall

  • 16:00 Return by the last boat from the upper pier

• 17:00 Arrival at Iriomote Station

• 18:30 Dinner

• 19:30

  • Workshop discussion on the use of eDNA for biodiversity management for the demands from local communities  (AJ-Core)

• 21:00  Closing the workshop

Researchers and Students who work on their research projects

• Emma Rossouw (Stellenbosch University, South Africa) (6 Jan - 7 Feb)

• Itchika Sivaipram  (Chulalongkorn University, Thailand) (16 - 25 Jan)

• Jasrul Dulipat  (Universiti Malaysia Sabah, Malaysia) (20 - 31 Jan)

• Leanne Frances Yee (University of Nottingham Malaysia, Malaysia) (3 - 14 Feb)

• Heng Wei Khang (Universiti Malaysia Terengganu, Malaysia) (3 - 14 Feb)

• Ndeye Codou Mbaye (Cheikh Anta Diop University, Senegal) (2 - 16 Feb)

• Jean Fall   (Cheikh Anta Diop University, Senegal) (2 - 16 Feb)

• Marizka Juliano (Gent University, Belgium) *Lab work supporter

• Bernadeth Grace Pananganan (University of the Ryukyus, Japan) *Lab work supporter

Symposium participants

• All researcher and students listed above

• Michio Kondo (Tohoku University, Japan)

• Yoshihisa Suyama (Tohoku University, Japan)

• Sioud Imane (Tohoku University, Japan)

• Yuki Minegishi (Tokyo University, Japan)

• Mohammad Basyuni  (Universitas Sumatera Utara, Indonesia)

• James Reimer  (University of the Ryukyus, Japan)

• Fabian Gosser (University of the Ryukyus, Japan)

• Ulla von Ammon  (University of the Ryukyus, Japan)

• Md. Mizanur Rahman (University of the Ryukyus, Japan)

• Keiko Shiromoto (University of the Ryukyus, Japan)

• Yukinobu Isowa  (TBRC, University of the Ryukyus, Japan)

• Tadashi Kajita (TBRC, University of the Ryukyus, Japan) 

• Tadashi Kajita (TBRC, University of the Ryukyus, Japan) 

• Yukinobu Isowa  (TBRC, University of the Ryukyus, Japan)

• Keiko Shiromoto  (TBRC, University of the Ryukyus, Japan, JSPS RPD)

• Bernadeth Grace Pananganan,  (TBRC, University of the Ryukyus, Japan), Research Assistant

• Md. Mizanur Rahman (University of the Ryukyus, Japan)

• Marizka Juliano (Gent University, Belgium) ,  Internship trainee at TBRC, University of the Ryukyus

• JSPS Core to Core Program.

(No. JPJSCCB20240006)  Program HP: https://www.jsps.go.jp/j-c2c/,  Project HP:  http://c2c.mangroves.info

• e-ASIA JRP  

(No. JPMJSC21E5). Program HP: https://www.the-easia.org/jrp/  Project HP (information) :  https://www.jst.go.jp/inter/english/program_e/multilateral_e/e_asia.html

• AJ-CORE JRP  

(No. JPMJSC21E5). Program HP:  https://www.jst.go.jp/inter/english/program_e/multilateral_e/aj-core.html

• JSPS KAKENHI - Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)) for FY2022 

No. (21KK0115)  Program HP: https://www.jsps.go.jp/j-grantsinaid/35_kokusai/04_kyoudoub/download.html

• Ocen Shot Project: "Holistic Genomic Approach to Asia-Pacific Marine Biodiversity" (Tohoku University)
  
  

• COI-Next Tohoku University
  Project HP (information) : https://www.naturepositive-hub.jp/  ; Japan Science and Technology Agency (COI-NEXT program, Grant number JPMJPF2206)